Research Project

Project Title:

IDENTIFICATION OF BIOMARKERS FOR EARLY DIAGNOSIS OF KIDNEY TRANSPLANT REJECTION

Project Type:

Translational research

Disease group(s):

Pediatric kidney transplantation

Project Summary:

Emerging data support the presence of biomarkers predictive of graft rejection in easily accessible biological fluids. Among these, extracellular vesicles (EVs) could represent a sensitive tool for monitoring renal graft status in kidney transplantation.
Objectives: An interdisciplinary approach will be used to correlate clinical, histological, immunological, biochemical and molecular data using a defined timeline monitoring model, in order to identify early predictive allograft rejection biomarkers. This study will lead to the development of novel methods to precociously diagnose, treat, and eventually prevent renal rejection, thus reducing the need of renal biopsy.
Research plan: We will consider 20 children transplanted in our UOSD Pediatric Nephrology Dialysis Transplantation Center of Padua from 2016 to the end of 2019. Urine, blood and tissue samples deriving from protocols biopsy are collected at 6-12-24 months after transplant. Multidisciplinary skills and application of innovative technologies (Fluorescent bead immunoassay, ddPCR, LC-MS/MS, Reverse Phase Protein Assay, FACs) will be applied to characterize the EVs origins and composition.

Lead principal investigator(s):

Luisa Murer, Padova
Paola Cogo, Udine

Co-investigator(s):

Susanna Negrisolo, Padova
Andrea Carraro, Padova
Elisa Benetti, Padova
Manuela Simonato, Udine

Project Period:

01/2019   -   12/2021

Sponsors:

Non-profit foundation

Project web page:

https://www.irpcds.org/partiti-nuovi-progetti-di-ricerca-innovativi-per-2-milioni-di-euro-irp-e-un-modello-conferma-il-sab/

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